hepatica individuals in these animals were immature (5–11 mm in length). The false-negative animals (5/7) were probably not detected because the F. The assay detected 100% of sheep with 1 fluke, 100% of cattle with 2 flukes, and 2 of 7 cattle with 1 fluke. hepatica ESA per milliliter of fecal supernatant. ![]() The MM3 capture ELISA assay had detection limits of 0.3 (sheep) and 0.6 (cattle) ng of F. hepatica infection (though in most cases harboring other helminths). Cattle feces were collected at the slaughterhouse from adult cows naturally infected with known numbers of flukes (from 1 to 154) or free of F. hepatica metacercariae and in some cases treated with triclabendazole at 14 wk postinfection (PI), and from uninfected control lambs. Sheep feces were obtained from a fluke-free herd (with most animals harboring other nematodes and cestodes), from lambs experimentally infected with 5–40 F. The specificity and sensitivity of the MM3 capture ELISA were assessed using feces from sheep and cattle. ![]() hepatica ESAs, which has previously been shown to be specific for the parasite. The mAb MM3 was produced by immunization of mice with a 7- to 40-kDa purified and O-deglycosylated fraction of F. A capture enzyme-linked immunosorbent assay (ELISA) using a new monoclonal antibody (mAb MM3) is reported for the detection of Fasciola hepatica excretory–secretory antigens (ESAs) in feces of infected hosts.
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